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NotificationsNotify me of updates to Comparison of DNA Damage and the Expression of Repair Related Molecules, Including DNA Polymerase-β, APE/ref-1, PCNA, and GADD45, in Human T and B Lymphocytes Exposed to Hydrogen Peroxide and Methyl Methanesulfonate (pp. 305-318)
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Comparison of DNA Damage and the Expression of Repair Related Molecules, Including DNA Polymerase-β, APE/ref-1, PCNA, and GADD45, in Human T and B Lymphocytes Exposed to Hydrogen Peroxide and Methyl Methanesulfonate (pp. 305-318) $100.00
Authors:  (Eunkyung Cho, Graduate School of Medicine, Korea Universty,So-Young Park, Environmental Toxico-Genomic and Proteomic Center, College of Medicine, Korea University, Donggeun Sul, Graduate School of Medicine, Korea Universty, Environmental Toxico-Genomic and Proteomic Center, College of Medicine, Korea University, Korea)
Abstract:
In this study, we evaluated the DNA damage and the expression of repair related
molecules, including DNA polymerase β, apurinic/apyrimidinic endonuclease /redox
factor-1 (APE/ref-1), proliferating cell nuclear antigen (PCNA), and growth arrest and
DNA damage (GADD45) in T and B human lymphocytes that were exposed to hydrogen
peroxide (H2O2) and methyl methane sulfonate (MMS). DNA damage was evaluated by
conducting a Comet assay of T and B cells that were exposed to H2O2 (25 and 50 μM)
and MMS (25 and 50 μM) for 5 min. The mean value of the Olive Tailmoment of the
control lymphocytes was 1.34 0.02 and 1.41 0.01 (p=0.084) in the T- and Blymphocytes,
respectively, which indicates that there was no significant difference in the
level of DNA damage that occurred. However, after being exposed to 25 μM and 50 μM
of H2O2, the mean values of the Olive Tailmoments of the T-lymphocytes were 1.92
0.11 (p=0.001) and 2.16 0.32 (p=0.001) whereas those of the B-lymphocytes were 1.98
0.11 (p=0.001) and 2.24 0.30 (p=0.001), which indicates that a significant level of
DNA damage occurred. In addition, after cells were exposed to 25 μM and 50 μM of
MMS, the mean values of the Olive Tailmoments of the T-lymphocytes were 1.80 0.09
(p=0.001) and 2.02 0.31 (p=0.001) whereas those of the B-lymphocytes were 1.88
0.14 (p=0.001) and 2.12 0.33 (p=0.001), which also indicates that a significant level of
DNA damage occurred. Furthermore, the level of DNA damage was found to be
significantly greater in B-lymphocytes than in T-lymphocytes. Taken together, these
results indicate that human B-lymphocyte may be a more sensitive target than Tlymphocytes
for the evaluation of DNA damage when conducting human biomonitoring.
When the expression of DNA polymerase β, APE/ref-1, GADD45 and PCNA was
evaluated, their relative intensities were found to increase significantly with increasing
concentrations of H2O2 and MMS in both T- and B-lymphocytes (p<0.05). In addition,
the relative intensity of GADD45 was significantly greater in the B-lymphocytes than in
T-lymphocytes.
In summary, a Comet assay showed that DNA damage occurred in response to H2O2
and MMS level in human T- and B-lymphocytes, and that this occurred in a dose
dependent fashion. In addition, the amount of DNA damage that occurred was greater in
B-lymphocytes than in T-lymphocytes, which indicates that human B-lymphocyte are
more sensitive target than T-lymphocytes for the evaluation of DNA damage when
conducting human biomonitoring. Futhermore, when the DNA repair enzyme and protein
expressions were compaired, GADD45 was found to be more sensitive to DNA damage
than DNA polymerase β, APE/ref-1, and PCNA in human T- and B-lymphocytes, which
could be useful in the evaluation of DNA damage as a result of genetic toxicants. 


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Comparison of DNA Damage and the Expression of Repair Related Molecules, Including DNA Polymerase-β, APE/ref-1, PCNA, and GADD45, in Human T and B Lymphocytes Exposed to Hydrogen Peroxide and Methyl Methanesulfonate (pp. 305-318)