Comparative Genomics for the Identification of RecQ DNA Helicases in Protozoan Parasites (pp. 135-156)
Authors: (Ma. del Socorro Charcas-López, Mavil López-Casamichana, César López-Camarillo and Laurence A. Marchat, Programa Institucional de Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatía-IPN, México, and others)
Abstract: DNA helicases are essential enzymes that catalyze the unwinding of DNA substrates
in an energy-dependent manner, participating in all the molecular processes involving
DNA. They are characterized by the presence of a conserved helicase domain with eight
well defined motifs including motif 0, as well as RecQ-Ct and HDRC domains. The
conservation of these domains through evolutionary scale can be successfully used in a
bioinformatics approach to identify new homologues in any genome sequence database.
In this chapter, we focused on the fully sequenced genome of Entamoeba histolytica,
Giardia lamblia, Trichomonas vaginalis and Plasmodium falciparum protozoan parasites,
which cause high morbidity and mortality in many developed and developing countries.
The identification of parasite RecQ DNA helicases and their further functional
characterization will provide new insights into the molecular biology of these major