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01.Small Molecule Promoted Feeder Free and Adherent Differentiation of Functional Neurons from Human Embryonic and Induced Pluripotent Stem Cells (pp. 1-8)
02.Induced Pluripotent Stem Cells for Peripheral Nerve Regeneration (pp. 39-50)
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Small Molecule Promoted Feeder Free and Adherent Differentiation of Functional Neurons from Human Embryonic and Induced Pluripotent Stem Cells (pp. 1-8) $45.00
Authors:  Danielle Drury-Stewart, Mingke Song, Osama Mohamad, Shan Ping Yu, and Ling Wei
Abstract:
While human embryonic stem (hES) and induced
pluripotent stem (hiPS) cells offer exciting prospects in the
fields of regenerative medicine and developmental biology,
efficient directed differentiation of these cells is still
difficult. Neural induction protocols often include
suspension culture or co-culture with other cell types,
introducing heterogeneity and complicating analysis. In
addition, expensive recombinant factors are often used over
processes that take weeks to complete, making such
experiments financially difficult. We have developed a fully
adherent and feeder free neural differentiation protocol
using small molecules such as dorsomorphin and common
medium supplements. Using this protocol, we obtain >90%
of cells developing into neural precursors, as measured by
nestin staining. Neurons derived from these precursors are
electrophysiologically active. After three weeks of terminal
differentiation, we obtain functional neurons which fire
high-amplitude action potentials upon depolarization. A
subset of neurons also fires repetitive trains. This protocol
offers a simpler and less expensive method for
investigations involving the differentiation of neural
precursors and neurons in culture.
Keywords: Embryonic stem cells, pluripotent 


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Small Molecule Promoted Feeder Free and Adherent Differentiation of Functional Neurons from Human Embryonic and Induced Pluripotent Stem Cells (pp. 1-8)