Nova Publishers
My Account Nova Publishers Shopping Cart
HomeBooksSeriesJournalsReference CollectionseBooksInformationSalesImprintsFor Authors
            
  Top » Catalog » Books » Biology » Biochemistry » Circular Dichroism: Theory and Spectroscopy Chapters » My Account  |  Cart Contents  |  Checkout   
Quick Find
  
Use keywords to find the product you are looking for.
Advanced Search
What's New? more
Circadian Clock: Regulations, Genetic and External Factors
$0.00
Shopping Cart more
0 items
Information
Shipping & Returns
Privacy Notice
Conditions of Use
Contact Us
Notifications more
NotificationsNotify me of updates to Investigation of Radiation-Induced Damages in DNA Structure by Circular Dichroism and UV Absorption Spectroscopy pp. 301-320
Tell A Friend
 
Tell someone you know about this product.
Investigation of Radiation-Induced Damages in DNA Structure by Circular Dichroism and UV Absorption Spectroscopy pp. 301-320 $100.00
Authors:  (S.V. Paston, O.A. Dommes, A.E. Tarasov, Saint Petersburg State University, Faculty of Physics, St. Petersburg, Russia)
Abstract:
It is well known that ionizing radiation causes modification and destruction of
nitrogenous bases in DNA molecule. There are also local breakages of hydrogen bonds
(partial denaturation) both in the lesion sites mentioned above and in other sites of the
macromolecule. To reveal the amount of some of these damages we applied circular
dichroism and UV absorption spectroscopy. Radiation-induced changes in DNA structure
influence its UV absorbtion spectrum in different ways: partial denaturation causes
hyperchromic effect, while destruction of the bases results in hypochromic shift. At the
same time both of them result in the same changes in DNA CD spectra: the decrease in
intensity. We attempted to segregate the described damages in DNA structure and studied
the influence of DNA ionic surroundings on the radiation effect. We have demonstrated
that the radiation efficiency of base destruction and partial denaturation increases with
decreasing concentration of NaCl in irradiated solution. The substitution of a portion of
Na+ ions on Mg2+ with the total ionic strength remaining constant (0.005 M) does not
influence the radiation efficiency. At the higher electrolyte concentration (3 M) the yield
of bases destruction is smaller in MgCl2 solutions than in NaCl ones, whereas the
secondary structure of irradiated DNA is destabilized stronger in MgCl2 solutions. Also
we have investigated the DNA spectral parameters in the presence of some biologically
active compounds in irradiated solutions such as aliphatic alcohols, catechin, epicatechin
and caffeine. It has been shown that all of these compounds reveal a radioprotective
properties. 


Available Options:
Version:
Special Focus Titles
01.Multicultural and Citizenship Awareness through Language: Cross Thematic Practices in Language Pedagogy
02.The Brainstem and Behavior
03.Sustainable Development: The Context of Use of Indigenous Plants for Local Economic Growth
04.Cancer versus Nutraceuticals
05.Fundamentals of Fuel Injection and Emission in Two-Stroke Engines
06.Free to Love: Schema Therapy for Christians
07.Textiles: Advances in Research and Applications
08.Cheese Production, Consumption and Health Benefits
09.Education in Lesotho: Prospects and Challenges
10.Work-Life Balance in the 21st Century: Perspectives, Practices, and Challenges
11.Electrical Measurements: Introduction, Concepts and Applications
12.Potassium Channels in Health and Disease

Nova Science Publishers
© Copyright 2004 - 2018

Investigation of Radiation-Induced Damages in DNA Structure by Circular Dichroism and UV Absorption Spectroscopy pp. 301-320