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Investigation of Radiation-Induced Damages in DNA Structure by Circular Dichroism and UV Absorption Spectroscopy pp. 301-320 $100.00
Authors:  (S.V. Paston, O.A. Dommes, A.E. Tarasov, Saint Petersburg State University, Faculty of Physics, St. Petersburg, Russia)
Abstract:
It is well known that ionizing radiation causes modification and destruction of
nitrogenous bases in DNA molecule. There are also local breakages of hydrogen bonds
(partial denaturation) both in the lesion sites mentioned above and in other sites of the
macromolecule. To reveal the amount of some of these damages we applied circular
dichroism and UV absorption spectroscopy. Radiation-induced changes in DNA structure
influence its UV absorbtion spectrum in different ways: partial denaturation causes
hyperchromic effect, while destruction of the bases results in hypochromic shift. At the
same time both of them result in the same changes in DNA CD spectra: the decrease in
intensity. We attempted to segregate the described damages in DNA structure and studied
the influence of DNA ionic surroundings on the radiation effect. We have demonstrated
that the radiation efficiency of base destruction and partial denaturation increases with
decreasing concentration of NaCl in irradiated solution. The substitution of a portion of
Na+ ions on Mg2+ with the total ionic strength remaining constant (0.005 M) does not
influence the radiation efficiency. At the higher electrolyte concentration (3 M) the yield
of bases destruction is smaller in MgCl2 solutions than in NaCl ones, whereas the
secondary structure of irradiated DNA is destabilized stronger in MgCl2 solutions. Also
we have investigated the DNA spectral parameters in the presence of some biologically
active compounds in irradiated solutions such as aliphatic alcohols, catechin, epicatechin
and caffeine. It has been shown that all of these compounds reveal a radioprotective
properties. 


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Investigation of Radiation-Induced Damages in DNA Structure by Circular Dichroism and UV Absorption Spectroscopy pp. 301-320