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Medicine Research Summaries (with Biographical Sketches). Volume 15
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01.Ecotoxicology of Free-Living Soil Nematode Caenorhabditis elegans pp. 1-52
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Antioxidant Response, Heavy Metal and Selenium Accumulation in Juvenile and Adult Procambarus clarkii of Both Sexes from Lake Trasimeno pp. 325-338 $100.00
Authors:  (Josef Martin Dörr Ambrosius, Nicole Pacini, Maria Cesarina Abete, Marino Prearo, Marzia Righetti, Concetta Antonia Elia, Department of Cellular and Environmental Biology, Laboratory of Ecotoxicology, Perugia University, Perugia, Italy, and others)
Abstract:
Procambarus clarkii was reported the first time, for the shallow Lake Trasimeno (Umbria, Italy), at the end of the nineties, but at the beginning of 2000 it became object of professional fishery and was sold in the local fish markets. The aim of this study was to evaluate the bioaccumulation of heavy metals, selenium and the antioxidant response in hepatopancreas of adult and juvenile crayfish of both sexes. Specimens were sampled in July 2007, in the post molt and prereproductive stage. Levels of cadmium, chromium, lead, selenium, glutathione and the enzymatic activities of glutathione S-transferase, glutathione peroxidase, glutathione reductase, and catalase were investigated for both life stages and sexes. The heavy metal accumulation of Cd, Cr, and Pb was very low and only slight differences were recorded between adults and juveniles of both sexes. Selenium accumulation was higher in males than in females and slight differences were noted between adults and juveniles. The European legislation fixed the limits for Cd, and Pb in crustacean products (EC Council Regulation 1881/2006) and according to this regulation the specimens showed metal concentrations under the limit of 0.5 mg/kg wet weight. These values indicate that Lake Trasimeno has a low contamination risk from these pollutants. The investigated antioxidant parameters showed levels, which were different in males and females and for juveniles and adults. The biochemical and chemical differences between sexes and life stages might be useful when P. clarkii is employed in biomonitoring programs and/or metal toxicity studies. 


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Antioxidant Response, Heavy Metal and Selenium Accumulation in Juvenile and Adult Procambarus clarkii of Both Sexes from Lake Trasimeno pp. 325-338