Nova Publishers
My Account Nova Publishers Shopping Cart
HomeBooksSeriesJournalsReference CollectionseBooksInformationSalesImprintsFor Authors
  Top » Catalog » Journals » Polymers Research Journal » Volume 3 Issue 3-4 Articles » My Account  |  Cart Contents  |  Checkout   
Quick Find
Use keywords to find the product you are looking for.
Advanced Search
What's New? more
Fetal Alcohol Syndrome: Recognition, Differential Diagnosis and Long-Term Effects
Shopping Cart more
0 items
Shipping & Returns
Privacy Notice
Conditions of Use
Contact Us
01.Physical–Chemical Properties of Natural Polymers – Potential Carriers and Delivery Systems of Biologically Active Substances for Human Applications (pp. 263-341)
Notifications more
NotificationsNotify me of updates to DEAE-Dextran and DEAE-Dextran-MMA Graft Copolymer for Nanomedicine
Tell A Friend
Tell someone you know about this product.
DEAE-Dextran and DEAE-Dextran-MMA Graft Copolymer for Nanomedicine $0.00
Authors:  YasuhikoOnishi, Yuki Eshita, and Masaaki Mizuno
2-diethylaminoethyl(DEAE)-Dextran is still an important substance for transfec-tion of nucleic acids into cultured mammalian cells by the reason of its safety owing to autoclave sterilization different from lipofection vectors. However,DEAE-Dextran may not be superior to lipofection vectors with cytotoxic and a transfection efficiency. A stable soap-less latex of 2-diethylaminoethyl(DEAE)-Dextran-methylmethacry-late(MMA) graft copolymer(DDMC)of a high transfection activity has been developed as Non-viral gene delivery vectors possible to autoclave at 121 for 15minutes. Transfection activity determined by the X-galstaining method show a higher value of 50 times or more for DDMC samples than for the starting DEAE-dextranhydrochlo-ride and a low cytotoxic is observed for DDMC. DDMC has been also observed to have a high protection facility for DNase degradation. The resulted DDMC on grafted MMA,to form a polymermicelle of Core-Shell particle, should become a stable latex with a hydrophilic-hydrophobic micro-separated-domain. The complex by DDMC/DNA may be formed initially on the stable spherical structure of the amphiphilicmicro-separated-domain of DDMC and have a good affinity to cell membrane for the endocytosis. The stronger infrared absorption spectrum shift to a high energy direction at around 3450 cm−one of the complexes between DNA and DDMC compared with DEAE-D extran may mean to form more compact structures not only by a coulomb force between the phosphoric acid of DNA and the diethyl-amino-ethyl (DEAE) group of DEAE-Dextran copolymer but also by a force from multi-inter-molecule hydrogen bond. It should conclude to DNA condensation by these inter-molecular multi-forces to be possible the higher transfection efficiency. The complex should be formed following Michaelis-Menten type equation such as complex = K1(DNA)(DDMC). It should be supported that DDMC has a strong adsorbing power with DNA because of not only its cationic property but also its hydrophobic bond and hydrogen bond. The high efficacy of this graft-copolymer autoclave-sterilized for transfection can make it a valuable tool for a safety gene delivery. 

Available Options:

  Open Access item.
  Click below PDF icon for free download.


This is an Open Access item. Click above PDF icon for free download.
Special Focus Titles
01.Violent Communication and Bullying in Early Childhood Education
02.The New Science of Curiosity
03.Cultural Considerations in Intervention with Women and Children Exposed to Intimate Partner Violence
04.Chronic Disease and Disability: The Pediatric Lung
05.Fruit and Vegetable Consumption and Health: New Research
06.Future Perspectives on Nigella sativa: Characterization and Pharmacological Properties
07.Wine and Winemaking: The Value and Efficiency
08.Impacts and Challenges in Oil Palm Cultivation and Downstream Applications of Biomass
09.Fire and the Sword: Understanding the Impact and Challenge of Organized Islamism. Volume 2
10.The Life and Times of the World's Most Famous Mathematicians
11.Innovation Processes in the Social Space of the Organization
12.High-Strength Steels: New Trends in Production and Applications

Nova Science Publishers
© Copyright 2004 - 2018

DEAE-Dextran and DEAE-Dextran-MMA Graft Copolymer for Nanomedicine