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01.Global Characterization and Genomic Stability of Human MultiStem, A Multipotent Adult Progenitor Cell pp. 17-28
02.Haematopoietic and Stromal Stem Cell Regulation by Extracellular Matrix Components and Growth Factors pp. 57-70
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Global Characterization and Genomic Stability of Human MultiStem, A Multipotent Adult Progenitor Cell pp. 17-28 $40.00
Authors:  Sherry Boozer, Nicholas Lehman, Uma Lakshmipathy, Brad Love, Amy Raber, Anirban Maitra, Robert Deans, Mahendra S. Rao and Anthony E. Ting
Abstract:
The therapeutic benefits of adult adherent stem cells are
currently being investigated in clinical trials for a variety of
diseases. Data from initial clinical studies are promising
and as a consequence of moving to later stage clinical
studies, have resulted in larger scale clinical-grade cell
production strategies. Therefore it becomes imperative to
examine the epigenetic flux and genomic stability of stem
cells in long-term culture to determine that minimal risk is
associated with these therapies.
Multipotent adult progenitor cells (MAPC) are an adherent
adult stem cell population that can be derived from bone
marrow and was the first of a class of adult stem cells that
have broad developmental potential both in vitro and in
vivo. Here, we report a panel of tests to characterize
MultiStem, a multipotent adult stem cell type based on
MAPC, and establish its genomic stability during culture
expansion.
A variety of techniques were employed that consisted of
miRNA expression to characterize and define the cell
population; chromosomal SNP analysis and G-banding to
determine karyotypic stability; and methylation pattern and
telomerase expression to examine potential changes in
epigenetic and chromosomal stability with prolonged in
vitro culture of cells. This panel of test was applied to
cultures at early isolation stages and compared to cultures
harvested at population doublings greater than those
reached in current MultiStem clinical trials. These tests also
provide a baseline for quality control of cells prepared from
various biological donor sources for subsequent large scale
propagation and preparation of cell banks for downstream
applications. 


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Global Characterization and Genomic Stability of Human MultiStem, A Multipotent Adult Progenitor Cell pp. 17-28